A 33-year-old heterosexual man, without any history of intravenous drug use, first diagnosed HIV infected in 2011, presented with a history of headache for 10 days, accompanied by fever and sudden onset of weakness on the left side of the body. He had history of skipping his ART drugs. On examination, he was disarthric and hemiplegic on the left side, with right-side facial palsy. CSF sample was sent for microbiological examination. Pic 1.
. Pic 1.
Identify the method of staining.
What is your diagnosis?
Staining method - Negative staining using modified India ink stain and nigrosin stain.
ReplyDeleteDiagnosis-
1. CSF microbial examination shows budding yeast cells surrounded by refractile clear capsules which indicate Cryptococcus neoformans.
2. The person is suffering from HIV which shows that he is immunocompromised.
3. Symptoms include fever, headache, left side hemiplegia which indicate meningitis.
Thus, the subject is diagnosed with cryptococcal meningitis which is potentially fatal in HIV infected people.
Staining method- negative staining by using dyes such as India ink or nigrosin.
ReplyDeleteIn which background stained black and yeast/fungus stand out in contrast as show in pic.1
Diagnosis-
The patient is suffering of cryptococcal meningitis caused by cryptococcus neoformans as shown in microbiological examination of his CSF.
Cryptococcus neoformans infect central nervous system (disarthric & hemiplegic on left side, right side facial palsy in patient) mostly in immunocompromised individual as such patient is diagnosed HIV infected.
Symptoms- fever, headache, weaknessetc.
Areeba Khan
ReplyDeleteRoll no. 16.
Staining method- negative staining by using dyes such as India ink or nigrosin.
In which background stained black and yeast/fungus stand out in contrast as show in pic.1
Diagnosis-
The patient is suffering of cryptococcal meningitis caused by cryptococcus neoformans as shown in microbiological examination of his CSF.
Cryptococcus neoformans infect central nervous system (disarthric & hemiplegic on left side, right side facial palsy in patient) mostly in immunocompromised individual as such patient is diagnosed HIV infected.
Symptoms- fever, headache, weaknessetc.
SAMRIDDHi GUPTA
ReplyDeleteROLL NO.71
Staining method- negative staining by using dyes such as India ink or nigrosin.
In which background stained black and yeast/fungus stand out in contrast as show in pic.1
Diagnosis-
The patient is suffering of cryptococcal meningitis caused by cryptococcus neoformans as shown in microbiological examination of his CSF.
Cryptococcus neoformans infect central nervous system (disarthric & hemiplegic on left side, right side facial palsy in patient) mostly in immunocompromised individual as such patient is diagnosed HIV infected.
Symptoms- fever, headache, weaknessetc.
SAMRIDDHi GUPTA
ReplyDeleteROLL NO. 71
Staining method - Negative staining using modified India ink stain and nigrosin stain.
Diagnosis-
1. CSF microbial examination shows budding yeast cells surrounded by refractile clear capsules which indicate Cryptococcus neoformans.
2. The person is suffering from HIV which shows that he is immunocompromised.
3. Symptoms include fever, headache, left side hemiplegia which indicate meningitis.
Thus, the subject is diagnosed with cryptococcal meningitis which is potentially fatal in HIV infected people.