The staining used in this case is Fluorescent staining. It uses fluorscent dye Auramine-O and phenol solution as primary stain. decolorizer is acid alcohol and 0.1% potassium permagnate is used as counter stain. Counter stain though not essential, is useful to quench background fluorescence. The mycolic acid in cell wall of mycobacteria have affinity for the fluorochromes thats the basis of using auramine-O.
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